Development and pilot validation of a cryopreservation protocol for species of the genus Arachis, based on the use of embryonic axes and the combined technique of desiccation–vitrification. The innovation standardises the stages of extraction, conditioning, controlled dehydration, vitrification, immersion in liquid nitrogen, thawing and in vitro regeneration, strengthening the safe and long-term conservation of peanut germplasm under ex situ conditions at INIAP.
Establishment of a pilot cryopreservation protocol for Arachis spp. through desiccation–vitrification of embryonic axes
Instituto Nacional de Investigaciones Agropecuarias
CIP - International Potato Center
Innovation is developed in the in vitro culture laboratory of the INIAP GeneBank located in Mejía, Pichincha, Ecuador.
Cesar Tapia
cesar.tapia@iniap.gob.ec
